Human Genetics & Embryology

Benoît Bertrand

Publications

• Review Article
  Are Genetic and Epigenetic Instabilities of Plant Embryogenic Cells a Fatality? The Experience of Coffee Somatic Embryogenesis
  Author(s): Hervé Etienne, Benoît Bertrand, Eveline Dechamp, Patrick Maurel, Frédéric Georget, Romain Guyot and Jean-Christophe BreitlerHervé Etienne, Benoît Bertrand, Eveline Dechamp, Patrick Maurel, Frédéric Georget, Romain Guyot and Jean-Christophe Breitler
  
  In plants, undifferentiated or totally differentiated cells can be easily in vitro cultured to generate undifferentiated embryogenic cells that can regenerate complete plants. This is the most spectacular expression of totipotency. Embryogenic cells represent a key material in plant biotechnologies as they are used in many processes aiming at asexual reproduction by somatic embryogenesis (SE), genetic transformation, protoplast regeneration and cryopreservation. Nevertheless, tissue culture systems that involve the acquisition of competence for totipotency and extensive cell division remain risky with respect to genome and epigenome instabilities. Particularly, the use of embryogenic cell suspensions has frequently been associated with an increased likelihood of genetic instability and somaclonal variation (SV) in the regenerated plants. SV is a major concern in all in vitro vegetativ.. Read More»
  DOI: 10.4172/2161-0436.1000136

  Abstract PDF