Research Article
Alternative Technology of Enzymatic Production of Eugenyl and Linalyl Acetate Esters
Author(s): Adriana BV, Bruna A, Glaucia F, Bruna MS Puton, Rogerio LC, Natalia P and Debora O VaninAdriana BV, Bruna A, Glaucia F, Bruna MS Puton, Rogerio LC, Natalia P and Debora O Vanin
Flavors production by enzymatic esterification is an important scientific and technological issue due to the numerous inconveniences related to the acid catalysis, mainly due to not be a clean technology. In this sense, this work used an alternative technology that describes the maximization of the enzymatic production of eugenyl acetate and linalyl acetate esters via enzymatic esterification of eugenol, a major component of clove oil (Caryophyllus aromaticus), and tertiary alcohol linalool, by using acetic anhydride and immobilized enzyme Novozym 435 as the catalyst in an organic solvent-free system. The best reaction condition with 100% of eugenyl acetate conversion was obtained at 60°C with an excess of acetic anhydride (1:5 molar ratio) and 10% of enzyme concentration (w/w in relation to the substrates). Higher yields of linalyl acetate (5.6%) were achieved with the e.. Read More»
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