Molecular Biology: Open Access

Carlos Francisco Sampaio Bonafe

Publications

• Research Article
  Epitope Mapping of Tobacco Mosaic Virus Capsid Protein: Prediction and Experimental Data from Spot Synthesis
  Author(s): Daniel Ferreira de Lima Neto, Clarice Weis Arns, Dagmar Ruth Stach-Machado, Fernando Rosado Spilki, Juliana Mattoso and Carlos Francisco Sampaio BonafeDaniel Ferreira de Lima Neto, Clarice Weis Arns, Dagmar Ruth Stach-Machado, Fernando Rosado Spilki, Juliana Mattoso and Carlos Francisco Sampaio Bonafe
  
  The immune system is a network of thousands of molecules, cells and regulatory factors that produce many interrelated responses. In this study, we used spot synthesis to map tobacco mosaic virus (TMV) epitopes in a mice animal model (Balb/c) and compared the results with those obtained using immunoinformatic prediction tools. Mice were inoculated with TMV and after immunization the sera were incubated with an array of overlapping pentadecapeptides that corresponded to the full sequence of the TMV capside protein (TMVcp) that had been synthesized on a cellulose membrane for spot synthesis analysis. Six linear epitopes were identified experimentally, as shown by the IgG-elicited immune responses in mice. The data for epitope prediction based on epitope databases agreed with the results obtained by spot synthesis results. Comparison of the findings for spot intensities and those obtained.. Read More»
  DOI: 10.4172/2168-9547.1000108

  Abstract PDF