Journal of Bioprocessing & Biotechniques

Carlos R. Soccol

Publications

• Research Article
  Process Development to Recover Pectinases Produced by Solid-State Fermentation
  Author(s): Daniel E. Rodríguez- Fernández, José A. Rodríguez León, Julio C. de Carvalho, Susan G. Karp, José L. Parada and Carlos R. SoccolDaniel E. Rodríguez- Fernández, José A. Rodríguez León, Julio C. de Carvalho, Susan G. Karp, José L. Parada and Carlos R. Soccol
  
   Leaching, or solid-liquid extraction, is the first step that must be done in the recovery process of a metabolite produced by Solid State Fermentation (SSF). In this work, the leaching of a Polygalacturonase (PG) produced by a strain of Aspergillus niger by SSF of citrus dried pulp was performed. A fractionated factor design 24-1 was developed to establish the influences of five factors: solid-liquid ratio, temperature, pH, agitation and surfactant addition (Tween 80). Agitation and surfactant addition effects were confounded by second order effects according to the fractionated factor design. Results showed that pH and surfactant addition did not influence the recovery process. The leaching process was characterized through the corresponding kinetic parameters for PG and total protein recovery, corresponding to first order kinetics. In the cas.. Read More»
  DOI: 10.4172/2155-9821.1000121

  Abstract PDF