Molecular and Genetic Medicine

Chae S

Publications

• Research Article
  Betaine Promotes LKB1-AMPK Activation Inhibits UVB-Mediated Senescence of Human Epidermal Keratinocytes Through Autophagy Induction
  Author(s): Kim KM, Im AR, Chae S and Kwon HJKim KM, Im AR, Chae S and Kwon HJ
  
  Betaine demonstrations antioxidative activity, enhances organic osmolytic activity and is an important cofactor in methylation. However, the main mechanisms betaine-induced autophagy in human dermal skin cells are not yet completely understood. Therefore, we hypothesized that betaine induces of autophagy. Thus, the autophagic effects exerted by betaine through activation of LKB1-AMPK signaling in human dermal fibroblasts (HDFs) and human epidermal keratinocytes (HEKs) were assessed. Betaine enhanced LKB1 and AMPK phosphorylation in HEKs, and LKB1, AMPK induced autophagy through mTOR downregulation. Beatine-induced autophagy was inhibited in cells transiently transfected with AMPK siRNA. Increased autophagosome activity was confirmed by LC3B-II formation and by increased perinuclear LC3B-II puncta in betaine-treated HEKs. according to our in vitro findings, and in vivo studies in HR-1 hai.. Read More»
  DOI: 10.4172/1747-0862.1000347

  Abstract PDF