Veterinary Science & Technology

Chitra K

Publications

• Research Article
  Individual and Multiplex PCR Assays for the Detection of Adventitious Bovine and Porcine Viral Genome Contaminants in the Commercial Vaccines and Animal Derived Raw Materials
  Author(s): Maroudam Veerasami, Chitra K, Mohana Subramanian B, Thamaraikannan P, Srinivasan VA and Dhinakar Raj GMaroudam Veerasami, Chitra K, Mohana Subramanian B, Thamaraikannan P, Srinivasan VA and Dhinakar Raj G
  
  Animal derived raw materials such as trypsin and Fetal Bovine Serum are used in vaccine manufacturing and pose the threat of introducing animal pathogens as contaminants into the final products. Thus screening for adventitious virus/ genome is part of quality control in manufacturing of biologicals. Various in-vitro and in-vivo detection assays have been developed for the detection of potential viral contaminants in vaccines. However, these assays are expensive, time consuming, labor intensive and incomplete limiting their ability to meet the increasing demands of the biological industry. Polymerase chain reaction technology scores over the in-vitro and in-vivo assays in speed, specificity, sensitivity and robustness of detection and can replace them in regular use. In the present study, a set of multiplex and individual PCRs were developed for the detecti.. Read More»
  DOI: 10.4172/2157-7579.1000179

  Abstract PDF