Cancer Science & Therapy

Cynthia A. Galindo

Publications

• Research Article
  Synergistic Effect Between Curcumin (diferuloylmethane) and Radiation on Clonogenic Cell Death Independent of p53 in Prostate Cancer Cells
  Author(s): Bijaya K. Nayak, Naveen K. Krishnegowda, Cynthia A. Galindo, Martin L. Meltz and Gregory P. SwansonBijaya K. Nayak, Naveen K. Krishnegowda, Cynthia A. Galindo, Martin L. Meltz and Gregory P. Swanson
  
  Radiotherapy is a common treatment for prostate cancer, but failure is observed 30 to 40% of the time. It is more common in patients with abnormal p53. The phytochemical diferuloylmethane (curcumin) a naturally occurring fl avinoid derived from the rhizome of Curcuma longa, shows potential radiosensitizing effects. In the present study, the effect of curcumin and radiation on cell viability, apoptosis and clonogenic cell death was examined in LNCaP (wild type p53) and PC3 (mutant p53) prostate cancer cells. The expression of p53 and p53 target genes was examined using RNase protection assay and Western blot analysis. Cell cycle and apoptosis was evaluated by fl ow cytometry. Cell viability and colony formation was examined using MTT assay and clonogenic assay respectively. The expression of p53, p21 and gadd45 increased in LNCaP cells after .. Read More»
  DOI: 10.4172/1948-5956.1000046

  Abstract PDF