Cancer Science & Therapy

Daniel M. Hayes

Publications

• Research Article
  Assessment of DNA Repair Mechanisms to Determine the Susceptibility of Non-small Cell Lung Cancer to Alkylating Agents
  Author(s): Ivette F. Emery, Chiara Battelli, Li Cai and Daniel M. HayesIvette F. Emery, Chiara Battelli, Li Cai and Daniel M. Hayes
  
  Background: The status of DNA repair systems impacts the sensitivity of tumors to chemotherapeutics. The anti-neoplastic activity of temozolomide, an alkylating agent, is affected by the enzyme O6-Methylguanine-DNA methyltransferase (MGMT), and by the mismatch repair system (MMR). Lack of MGMT makes a tumor susceptible to temozolomide provided MMR is functional. A non-functional MMR renders the tumor resistant to alkylating agents. We assayed MGMT and MMR in order to estimate the proportion of patients affected by NSCLC who may derive benefit from temozolomide, an alkylating agent not currently used in lung cancer. Method: MGMT and MMR were assayed by promoter methylation testing and by microsatellite instability testing, respectively, on 96 paraffin-embedded NSCLC samples. Methylation testing, which detects gene silencing, was conducted by methylation-specific PCR of the MGMT promote.. Read More»
  DOI: 10.4172/1948-5956.1000067

  Abstract PDF