Journal of Bioengineering & Biomedical Science

Daniel Mueller

Publications

• Research Article
  Organotypic Cultures of Hepg2 Cells for In Vitro Toxicity Studies
  Author(s): Daniel Mueller, Anika Koetemann and Fozia NoorDaniel Mueller, Anika Koetemann and Fozia Noor
  
  Tissue engineering of human liver cells in a three dimensional cell culture system could improve pharmacological studies in terms of drug metabolism, drug toxicity or adverse drug effects by mimicking the in vivo situation. In this study, we produced 3D organotypic cultures of HepG2 cells using the hanging drop method. 250 – 8000 seeded cells formed organotypic cultures within 2–3 days which increased in size during the first week. Viability and metabolic parameters (glucose, lactate) were analyzed during almost three weeks of cultivation. Liver specific albumin production was higher in the organotypic cultures as compared to both monolayer and collagen-sandwich cultures. Amino acid quantification revealed high production of glutamate as well as uptake of glutamine, alanine and branched-chain amino acids. CYP1A induction capacity was significantly i.. Read More»
  DOI: 10.4172/2155-9538.S2-002

  Abstract PDF