Molecular Biomarkers & Diagnosis

Daniel Seifu

Publications

• Research Article
  Application of Proteases as Molecular Imaging Tools for Early Gastric Cancer Detection: In Vitro and Ex Vivo Investigation
  Author(s): Wajana Lako Labisso and Daniel SeifuWajana Lako Labisso and Daniel Seifu
  
  Background: Substantial miss-rate of gastric cancer by conventianl endoscope was reported, indicating the importance of novel biomarkers for early detection of precancerous and cancerous lesions. Objective: To identify potential biomarker molecules for early gastric cancer detection and evaluation of their application for molecular in vivo imaging. Method: Besides the CEA-TAG+/- mouse model, 3 human and 2 murine gastric cancer cell lines were applied in this study. Cells lines were cultured at standard conditions. To determine the expression pattern of the targets, total RNA was extracted from cultured cells; cDNA was synthesised and qPCR was performed using spcefic primers. The murine stomach was removed and washed with PBS. The stomach was macroscopically categorized into tumor region and normal tissues... Read More»
  DOI: 10.4172/2155-9929.1000366

  Abstract PDF