Veterinary Science & Technology

Daniel Shiferaw

Publications

• Review Article
  Application of Molecular Diagnostic Techniques for the Detection of E. coli O157:H7: A Review
  Author(s): Nateneal Tamerat, Yimer Muktar and Daniel ShiferawNateneal Tamerat, Yimer Muktar and Daniel Shiferaw
  
  Shiga-toxin producing Escherichia coli are considering as important emerging food-born bacterial pathogens. Among these kinds of Escherichia coli, serotype O157:H7 is the cause of sporadic and epidemic human cases of hemorrhagic colitis, which can lead to severe life threatening hemolytic uremic syndrome. Majority of cases are associated with ingestion of food or water contaminated with Escherichia coli O157:H7. This serotype is identified in 1982, and afterward the different pathogenic genes responsible for its virulence are sequenced. As the organism has been involved in many outbreaks of disease in human and animals, it is essential to develop a rapid, reliable and reproducible method of detection. In this review, different deoxyribonucleic acid based genotyping and detection methods are discussed. Genomic techniques like those that pulse field gel electr.. Read More»
  DOI: 10.4172/2157-7579.1000362

  Abstract PDF