Veterinary Science & Technology

Danielle R. O’Brien

Publications

• Research Article
  Gemcitabine Degradation in Whole Blood from Humans, Dogs, Cats, and Horses
  Author(s): Danielle R. O’Brien, Teri A Guerrero, Sara Allstadt Frazier, Robert B Rebhun, Katherine A Skorupski and Carlos O Rodriguez JrDanielle R. O’Brien, Teri A Guerrero, Sara Allstadt Frazier, Robert B Rebhun, Katherine A Skorupski and Carlos O Rodriguez Jr
  
  The purpose of this in vitro study was to compare the degradation of gemcitabine (2’, 2’-difluorodeoxycytidine, dFdC), in Fresh Whole Blood (FWB) from humans, dogs, cats, and horses. A better understanding of the comparative degradation of gemcitabine may aid in the optimal design of therapeutic regimens in veterinary species. Fresh whole blood from humans, dogs, cats, and horses was spiked with dFdC and plasma was analyzed for dFdC and 2’, 2’-difluorodeoxyuridine (dFdU) by high performance liquid chromatography. In these species, there was an initial rapid degradation of dFdC with a concomitant proportional increase in dFdU. Degradation of gemcitabine appeared similar in humans, dogs, and horses (p>0.05) whereas metabolism was slower in the cat than human (p=0.014), dog (p=0.010), or horse (p=0.0015). Based on these in vitro findings, dosing schemes for hum.. Read More»
  DOI: 10.4172/2157-7579.1000119

  Abstract PDF