Veterinary Science & Technology

David J. Wilson

Publications

• Research Article
  Johne’s disease, Mycoplasma and BVD in Utah-Bulk Tank Milk Testing and Comparison to Previous Regional Prevalence and Individual Herd Results over Time
  Author(s): David J. Wilson, Kerry A. Rood, Jennifer Bunnell, Chelsea Whitehouse, Todd M. Byrem and Gregory M. GoodellDavid J. Wilson, Kerry A. Rood, Jennifer Bunnell, Chelsea Whitehouse, Todd M. Byrem and Gregory M. Goodell
  
  Dairy herd-level prevalence of Mycobacterium avium subsp. paratuberculosis (MAP), causative agent of Johne’s disease (JD), Mycoplasma spp., and Bovine Viral Diarrhea (BVD) virus were estimated in Utah and surrounding states and compared to previous surveillance results. Milk was collected at 3-4 day intervals on 5 dates (duplicate samples) from each bulk tank on participating farms, samples analyzed separately. One frozen sample was shipped to a laboratory for JD/MAP testing with ELISA and real-time PCR, the other paired sample was shipped to another laboratory for mycoplasma and BVD testing. Mycoplasma was cultured on modified Hayflick medium, standard methods; BVD testing was real-time, RT-PCR. 151/209 (72%) eligible dairy farms participated. Farms detected positive (some had multiple diseases) were: 58 JD (38%); 4 mycoplasma (3%); 14 BVD (9%). Sensitivity of testing was: 284/.. Read More»
  DOI: 10.4172/2157-7579.1000182

  Abstract PDF