Molecular Biology: Open Access

David Meyers

Publications

• Research Article
  Regulation of Myf5 Early Enhancer by Histone Acetyltransferase p300 during Stem Cell Differentiation
  Author(s): Tanja Francetic, Melanie Le May, Munerah Hamed, Hymn Mach, David Meyers, Philip A Cole, Jihong Chen and Qiao LiTanja Francetic, Melanie Le May, Munerah Hamed, Hymn Mach, David Meyers, Philip A Cole, Jihong Chen and Qiao Li
  
  Skeletal myogenesis is an intricate process coordinated temporally by multiple myogenic regulatory factors (MRF) including Myf5, which is the first MRF expressed and marks the commitment of skeletal muscle lineage. The expression of Myf5 gene during early embryogenesis is controlled by a set of enhancer elements, and requires the histone acetyltransferase (HAT) activity of transcriptional coactivator p300. However, it is unclear as to how different regulatory signals converge at enhancer elements to regulate early Myf5 gene expression, and if p300 is directly involved. We show here that p300 associates with the Myf5 early enhancer at the early stage of stem cell differentiation, and its HAT activity is important for the recruitment of β-catenin to this early enhancer. In addition, histone H3-K27 acetylation, but not H3-K9/14, is intimately connected to the p300 HAT activ.. Read More»
  DOI: 10.4172/2168-9547.1000103

  Abstract PDF