Journal of Tissue Science and Engineering

David Oehme

Publications

• Research Article
  Radiological, Morphological, Histological and Biochemical Changes of Lumbar Discs in an Animal Model of Disc Degeneration Suitable for Evaluating the Potential Regenerative Capacity of Novel Biological Agents.
  
  Author(s): Peter Ghosh, David Oehme, Tony Goldschlager, Susan Shimon, Jiehua Wu, Stephen Stuckey, Mark Williamson, Jeffrey Rosenfeld and Graham JenkinPeter Ghosh, David Oehme, Tony Goldschlager, Susan Shimon, Jiehua Wu, Stephen Stuckey, Mark Williamson, Jeffrey Rosenfeld and Graham Jenkin
  
  Circumferential tears of the Annulus Fibrosus (AF) are frequently observed pathological features of degenerate lumbar discs and have been associated with vascular propagation and the generation of low back pain. In order to evaluate the potential of novel biological agents to repair annular defects and arrest disc degeneration we required an animal model that would permit injection of cells or their cryoprotectant into adjacent lumbar discs of the same animal. Three lumbar discs (L2/3, L3/4 and L4/5) of 6 sheep were subjected to a peripheral lateral annular surgical incision. The adjacent uninjured lumbar L1/2 and L5/6 discs served as uninjured controls. After three months the spines were radiographed and disc height indices (DHI) calculated and Pfirrmann disc degeneration scores determined from MRI spinal images. Isolated lumbar discs were analysed morphologically, h.. Read More»
  

  Abstract