Journal of Bioprocessing & Biotechniques

De la Puente R

Publications

• Research Article
  A New Procedure for DNA Isolation from Saliva Samples and Comparative Analysis of Quality Indicators
  Author(s): Carrillo Avila JA, De la Puente R, Pinaglia Tobaruela G, Aguilar Quesada R, Rueda Medina B, Correa Rodriguez M and Espin Vallejo LCarrillo Avila JA, De la Puente R, Pinaglia Tobaruela G, Aguilar Quesada R, Rueda Medina B, Correa Rodriguez M and Espin Vallejo L
  
  Background: Large population-based studies involving thousands of participants are needed for research on genetic diseases and epidemiologic studies. Saliva samples are a non-invasive and efficient DNA source for mass collection. The establishment of new optimized DNA isolation procedures from saliva and the determination of the most effective quality indicator are essential for this purpose. Methods: DNA was extracted from 112 saliva samples utilizing a novel method. Samples were pre-treated with Protease for 1 h at 56°C, and reagents from a kit for blood samples were used in the Chemagic MSM-I automated instrument with a specifically designed saliva protocol for the Chemagic software. DNA quality was estimated by spectrophotometry, fluorometry, qPCR, SPUD assay and the Agilent 2200 Tape Station. Results: An a.. Read More»
  

  Abstract PDF