Cancer Science & Therapy

Del Campo A

Publications

• Research Article
  Recovery HLA-A2 and Beta2-microglobulin Expression in Tumor Cells Using Viral Vectors
  Author(s): Carretero FJ, Del Campo A, Zinchenko S, Garrido F and Aptsiauri NCarretero FJ, Del Campo A, Zinchenko S, Garrido F and Aptsiauri N
  
  Background: Tumor elimination and the success of cancer immunotherapy depend on the proper expression of HLA class I complex (HLA-I) required for the presentation of tumor-associated peptides to cytotoxic T-lymphocytes. Tumors escape immune attack by losing HLA-I expression, often due to irreversible genetic/chromosomal alterations, including mutations in beta2-microglobulin (B2M) or lack of HLA-A2 allele due to a haplotype loss. The introduction of these genes and re-expression of the missing HLA-I specificity on the tumor cell surface is an attractive strategy to induce tumor rejection by T-lymphocytes. Methods: Using genomic HLA-I typing and gene sequencing we determined HLA-I phenotypes and alterations in different human tumor cell lines previously characterized in our laboratory. We used adeno- and adeno-associated viruses to reconstitu.. Read More»
  DOI: 10.4172/1948-5956.1000483

  Abstract PDF