Journal of AIDS & Clinical Research

Dino Bee Aik Tan

Publications

• Research Article
  Characteristics of Natural Killer Cells in Malaysian HIV Patients Presenting with Immune Restoration Disease After ART
  Author(s): Dino Bee Aik Tan, Yean Kong Yong, Hong Yien Tan, Martyn French, Adeeba Kamarulzaman and Patricia PriceDino Bee Aik Tan, Yean Kong Yong, Hong Yien Tan, Martyn French, Adeeba Kamarulzaman and Patricia Price
  
  Objectives: Natural killer (NK) cell function was investigated in Malaysian HIV patients beginning antiretroviral therapy (ART) with advanced immunodeficiency. Some patients experienced immune restoration disease (IRD) presenting as exacerbations of pre-existing infections. Whilst most IRD are attributed to interferon-gamma (IFN?) produced by T-cells, NK cells may also contribute. Methods: Blood leukocytes were collected prospectively from 100 HIV patients over 1 year on ART, plus 36 healthy controls. Eleven patients who experienced an IRD and 14 matched controls were assayed. Cells producing IFN? were quantitated by ELISpot after stimulation with an NK target (K562 cells) or antigens from pathogens associated with the IRD. NK cell subsets, CD16 and perforin expression were determined by flow cytometry Results: NK cell IFN? responses were lower in HIV patients at base.. Read More»
  DOI: 10.4172/2155-6113.1000102

  Abstract PDF