Tanzania
Research Article
Alpha-Defens in 5 Expression is Regulated by microRNAs in the Caco-2 Intestinal Epithelial Cell Line
Author(s): Donald R B Miles, Jun Shen, Alice Y. Chuang, Fenshi Dong, Feng Wu and John KwonDonald R B Miles, Jun Shen, Alice Y. Chuang, Fenshi Dong, Feng Wu and John Kwon
Abstract Background: In inflammatory bowel disease (IBD), an inappropriate immune response leads to chronic mucosal inflammation. This response may be partly due to dysregulation of defensins, which are endogenously produced antimicrobial peptides. This study determined whether microRNAs (miRNAs) regulate α-defensin 5 (DEFA5), which could further implicate both in IBD pathogenesis. Methods: Induction of DEFA5 mRNA and protein expression was determined in Caco-2 cells. An in silico analysis identified putative miRNA binding sites of DEFA5. Expression of these miRNAs was assessed in Caco-2 cells. Regulation of DEFA5 expression by these miRNAs was measured by luciferase assays. Caco-2 cells were transfected with miR-124 and miR-924 mimics, and DEFA5 mRNA and protein expression was measured. Results: DEFA5 mRNA and protein expression was inducible in Caco-2 cells. Fifteen putative m.. Read More»
DOI:
10.4172/2476-1958.1000105
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