Karolinska Institute,
Hospital Huddinge, S-141 86 Stockholm
Sweden
Research Article
Isolation and Characterization of the SSEA-1+ Progenitor Cells from the
Human Embryonic Heart
Author(s): Elzafir Elsheikh, Rami Genead, Christian Danielsson, Eva Wärdell, Agneta Andersson, Anders Kjaeldgaard, Erik Sundström, Tolga Sutlu, Karl-Henrik Grinnemo and Christer SylvénElzafir Elsheikh, Rami Genead, Christian Danielsson, Eva Wärdell, Agneta Andersson, Anders Kjaeldgaard, Erik Sundström, Tolga Sutlu, Karl-Henrik Grinnemo and Christer Sylvén
Background: The optimal stem cell source for regenerative therapy of the failing heart has not been settled. Embryonic stem cells, bone marrow derived cells, skeletal myoblasts, as well as cells derived from adult cardiac biopsies have been explored. Here we investigated the option to generate heart progenitor cells from the early human embryonic heart (hEHPCs) for the treatment of acute myocardial infarction. Methods: Two hearts (8 and 8.5 gestational weeks) from human abortion material were used for the clonal expansion of hEHPCs after stage specific embryonic antigen 1 (SSEA-1) enrichment by magnetic beads isolation. GFP-transduced hEHPCs- were transplanted into 18 SCID mice intramyocardially after induction of myocardial infarction. Hearts were harvested every 72 hours. Results: In this study we succeeded to isolate clones of hEHPCs with similar characteristics twice from two .. Read More»
Journal of Cytology & Histology received 2476 citations as per Google Scholar report
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