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Research Article
Bioassay of Toxoplasma Gondii from Apparently Healthy Pigs Slaughtered in Addis Ababa Abattoir, Ethiopia
Author(s): Endrias Zewdu Gebremedhin, Mulisa Megerssa Kebeta, Mebratu Asaye, Hagos Ashenafi, Vincenzo Di Marco and Maria VitaleEndrias Zewdu Gebremedhin, Mulisa Megerssa Kebeta, Mebratu Asaye, Hagos Ashenafi, Vincenzo Di Marco and Maria Vitale
Toxoplasmosis is an infectious disease caused by the protozoan parasite Toxoplasma gondii that infects a wide range of animals, including man and birds. The objective of the study was to isolate T. gondii in pigs slaughtered at the Addis Ababa abattoir. A total of 290 sera were examined for T. gondii antibodies (IgG) using the Direct Agglutination Test (DAT) of which 111 pigs (38.28%; 95% confidence interval [CI]: 32.65%-44.14%) were seropositive. Hearts of fifty seropositive pigs and two pooled pig heart samples (portion of 4 pig hearts per pool) were used for the study. For the isolation of T. gondii, 50 g heart tissue was ground, digested with acid-pepsin and inoculated intraperitoneally into mice (5 mice/sample). Viable T. gondii was isolated from hearts of 24 of 50 (48%; 95% CI: 33.7%-62.6%) seropositive pigs and from the two pooled seronegative pig hearts. All isolates were asym.. Read More»
DOI:
10.4172/2157-7579.1000258
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