Journal of AIDS & Clinical Research

Fabian Camacho

Publications

• Research Article
  Comparisons of VLP-Based ELISA, Neutralization Assays with Native HPV, and Neutralization Assays with PsV in Detecting HPV Antibody Responses in HIV-Infected Women
  Author(s): Ping Du, Sarah Brendle, Janice Milici, Fabian Camacho, John Zurlo, Neil Christensen and Craig MeyersPing Du, Sarah Brendle, Janice Milici, Fabian Camacho, John Zurlo, Neil Christensen and Craig Meyers
  
  Objective: Human papillomavirus (HPV)-associated cancers are important public health problems in HIV-infected people. Assays based on HPV virus-like particles (VLP) and pseudoviruses (PsV) are commonly used to examine HPV antibody responses in HIV-infected people, but neutralization assays with native HPV have not been utilized and a comparison of these three assays is lacking. We evaluated the agreement of assays using VLP, native HPV and PsV in detecting HPV16 and 18 antibodies in HIV-infected women. Methods: The VLP-based ELISA (VLP-ELISA) was used to detect antibody responses to HPV16 and 18 and cottontail rabbit papillomavirus (CRPV) VLP antigens. Neutralization assays with native HPV (NA-HPV) and with PsV (NA-PsV) were conducted to examine HPV16 or 18 neutralizing antibodies. Intra class correlation coefficients (ICC) and kappa coefficients were used t.. Read More»
  DOI: 10.4172/2155-6113.1000433

  Abstract PDF