China
Research Article
Effect of Vanadium on Splenocyte Apoptosis in Broilers
Author(s): Cui Wei, Cui Hengmin, Peng Xi, Fang Jing, Liu Xiaodong and Wu BangyuanCui Wei, Cui Hengmin, Peng Xi, Fang Jing, Liu Xiaodong and Wu Bangyuan
A total of 420 one-day-old healthy broilers were divided into six groups. There were 70 broilers in each group. The broilers were fed on a corn-soybean basal diet as a control diet (vanadium 0.073 mg/kg) or the same diet amended to contain 5 mg/kg, 15 mg/kg, 30 mg/kg, 45 mg/kg and 60 mg/kg vanadium supplied as ammonium metavanadate for 42 days. When compared with those of the control group, the mitochondrial injury of splenocytes and more apoptotic splenocytes with condensed chromatin with C-shaped, horseshoe-like, petal-shaped or crescent were ultrastructurally observed in the spleen in the 30 mg/kg, 45 mg/kg and 60 mg/kg groups. As measured by flow cytometry (FCM), the number of apoptotic splenocytes was significantly increased in the 15 mg/kg, 30 mg/kg, 45 mg/kg and 60 mg/kg groups. Immunohistochemical tests showed that number of positive splenocytes containing Bax and caspase-3 pr.. Read More»
DOI:
10.4172/2161-0444.1000115
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