Molecular Biology: Open Access

Fathi Hassan

Publications

• Research Article
  Improved Protocol for Agrobacterium-Mediated Transformation of Pea (Pisum sativum)
  Author(s): Mitra Aftabi, Alemayehu Teressa Negawo and Fathi HassanMitra Aftabi, Alemayehu Teressa Negawo and Fathi Hassan
  
  Pea is one of the most important legume crops whose production is constantly threatened by field and storage pests and diseases. Developing insect resistant transgenic pea plants through Agrobacterium-mediated transformation is a promising solution to maintain crop yield. However, the transformation efficiency is still low. Therefore, there was an attempt to enhance transformation efficiency by optimizing infection time, co-cultivation period and in vitro regeneration system. Transformation was performed using segments of embryonic axes from mature pea seeds (Pisum sativum L. cv. Sponsor). The segments were inoculated with the hyper virulent EHA105 strain of Agrobacterium tumefaciens. The Agrobacterium strain harboured a binary vector pGII35S containing the bar gene which confers resistance to phosphinotricin. The selection medium contained P2 medium with increasing concentrations of .. Read More»
  DOI: 10.4172/2168-9547.1000202

  Abstract PDF