Cameroon
Research Article
Production and Partial Characterization of an Extracellular Thermophile Alkaline Protease from a Selected Strain of Bacillus sp Isolated from Abattoir Soil in the North Region of Cameroon
Author(s): Frédéric Tavea, Bertrand Tatsinkou Fossi, Noubouche Tefounou Fabrice, Leopold Tatsadjieu Ngoune and Robert NdjouenkeuFrédéric Tavea, Bertrand Tatsinkou Fossi, Noubouche Tefounou Fabrice, Leopold Tatsadjieu Ngoune and Robert Ndjouenkeu
The present study aims at screening and isolates thermostable alkaline protease producing bacteria from soil sample in the northern part of Cameroon, and characterizes their enzyme. Samples of soil were collected from four (04) North Cameroonian towns (Ngaoundere, Garoua, Figuil and Maroua). Six (06) isolates showed important proteolytic activity on skim milk agar. The strain N2.3.5 from Ngaoundere showed the largest hydrolysis halo (24 mm) and was selected for protease production. The partial identification of strain N2.3.5 through morphological and biochemical characterization enabled us to classify it as belonging to the genus Bacillus sp. The growth of the strain N2.3.5 and its enzyme production profile showed that the growth of the bacteria was a diauxic type, and that the protease production was not directly associated with the microbial gro.. Read More»
DOI:
10.4172/2155-9821.1000279
Journal of Bioprocessing & Biotechniques received 3351 citations as per Google Scholar report