Department of Zoology, Mizoram University,
Tanhril, Aizawl-796 004, Mizoram
India
Research Article
Alteration in the Doxorubicin-Induced DNA Damage in Cultured V79 Cells by Aegle marmelos (L.) Correa (Bael): A Comet Assay Study
Author(s): Ganesh Chandra JagetiaGanesh Chandra Jagetia
The clinical applicability of wide spectrum chemotherapeutic drug, doxorubicin is limited due to induction of severe cardiomyopathy resulting from DNA damage. The present study was aimed to evaluate the protective effect of bael (Aegle marmelos) extract (AME) on the doxorubicin-induced molecular DNA damage in V79 cells. V79 (Chinese hamster lung fibroblasts) cells were treated with 0 or 25 μg/ml AME before exposure to 0, 1, 2.5, 5, 10, 25 or 50 μg/ml doxorubicin DOX. The DNA damage was studied at different post-doxorubicin treatment times using single cell gel electrophoresis. Doxorubicin caused a maximum DNA damage at 1 h post-DOX treatment indicated by a highest Olive Tail Movement (OTM) and tail DNA, whereas treatment of V79 cells with 25 μg/ml AME enhanced DNA repair at all assessment times with a maximum repair up to 6 h which did not alter thereafter. In anothe.. Read More»
DOI:
10.4172/2376-0281.1000190
International Journal of Neurorehabilitation received 1078 citations as per Google Scholar report