Journal of Cytology & Histology

García-Medrano B

Publications

• Research Article
  The Role of the Basal Lamina in Nerve Regeneration
  Author(s): García-Medrano B, Pérez CS, Sanz PB, García MG, Martín-Ferrero M and Rodríguez MJGGarcía-Medrano B, Pérez CS, Sanz PB, García MG, Martín-Ferrero M and Rodríguez MJG
  
  Introduction: In the context of axon regeneration, certain molecules containing the basal lamina, particularly fibronectin, laminin and heparin sulfate promote axonal elongation in vitro and in vivo. This structure could lead the axons to distal stump in nerve defect repair. Material and Methods: In twenty male Wistar rats, a critical defect of 15 mm was created in the sciatic nerve, which was bridged by an acellular muscle graft, which were obtained from the gluteus medius muscle of two donor rats, and chemically treated to cellular extraction by protocol consisted of 7 h in distilled water changed three times every 2-3 h, one night in Triton detergent, and 24 h in deoxycholate. After 90 days, the explanted pieces of sciatic nerve with grafts were carved and cut into seven pieces equally in all animals; pieces were processed in resin (toluidine blue staining) and also for paraffin em.. Read More»
  DOI: 10.4172/2157-7099.1000438

  Abstract PDF