Journal of Nephrology & Therapeutics

Glinkina KA

Publications

• Research Article
  Production of Thrombin Complexes with DNA Aptamers Containing G-Quadruplex and Different Duplexes
  Author(s): Vera A. Spiridonova, Glinkina KA, Gainutdinov AA and Arutyunyan AMVera A. Spiridonova, Glinkina KA, Gainutdinov AA and Arutyunyan AM
  
  Despite major advances in the understanding of the molecular mechanisms of thrombosis and the development of effective thrombolytic agents, arterial thrombosis remains a formidable clinical problem. A new class of direct thrombin inhibitors (aptamers), has been described recently. Aptamers, single-stranded oligonucleotides with a length of 30-60 nucleotides, exhibit high affinity and specificity towards any defined recognition target (protein). Aptamers are antibody analogs in terms of both specificity and affinity, with an apparent advantage of the former to being reproduced by anautomated chemical synthesis. Aptamers are routinely selected by SELEX technology (Systematic Evolution of Ligands by Exponential enrichment), which is thoroughly discussed elsewhere. The DNA aptamers to thrombin have a very highly ordered tertiary structure (G-quadruplex). A great number of the DNA aptamers.. Read More»
  DOI: 10.4172/2161-0959.1000149

  Abstract PDF