Central Research Laboratory, Industrial Biotechnology, GITAM Institute of Technology,
Visakhapatnam-530045, Andhra Pradesh
India
Research Article
Optimization of Chemical Parameters for the Production of Citric acid using Box-Behnken Design
Author(s): Gopinadh R, Ayyanna C, Ramakrishna Ch, Narayana Saibaba KV, Ravi vittal K and Jagadhi RGopinadh R, Ayyanna C, Ramakrishna Ch, Narayana Saibaba KV, Ravi vittal K and Jagadhi R
Statistical experimental design was employed for the improvement of citric acid production from Madhuca indica through submerged fermentation process using mutant fungi Aspergillus niger MTCC 282. Efficient production of citric acid by mutant Aspergillus niger MTCC 282 grown on a sugar rich byproduct requires the supplementation of the optimized quantities of nutrients. Optimization of chemical parameters for the production of citric acid was done by Box-Behnken design. Box-Behnken design was used to statistically compare different combinations of four different nutrients, namely Ammonium sulphate ((NH4)2SO4), Magnesium (Mg2+), Ethylene diamine tetra acetic acid (EDTA) and Potassium phosphate (KH2PO4), for the maximum production of citric acid. The optimum nutrient concentrations obtained usin.. Read More»
DOI:
10.4172/2155-9821.1000244
Journal of Bioprocessing & Biotechniques received 3351 citations as per Google Scholar report