Journal of Cytology & Histology

Guohua Jin

Publications

• Research Article
  IGF2 Regulates Neuronal Differentiation of Hippocampal Radial Glial Cells In Vitro
  Author(s): Xuelei Tao, Guohua Jin, Linqing Zou, HaoMing Li and Jianbing QinXuelei Tao, Guohua Jin, Linqing Zou, HaoMing Li and Jianbing Qin
  
  We previously observed that radial glial(RG)-like cells showed larger soma, thicker and longer neuritis in vivo(i.e., in rats) after fimbria-fornix (FF) transection, and in vitro RG-like cells showed the same results after the application of the extract from FF-transected hippocampus. In the present study, RG-like cells cultured in 24-well plates supplemented with 5% extract from FF-transected hippocampus were more likely to differentiate into neurons, compared with a normal group. After receiving the insulin-like growth factor 2 (IGF2) gene and knockdown or over expression viruses, the influence of IGF2 on the differentiation of RG-like cells into neurons and the expression of IGF2 were measured. Then, real-time polymerase chain reaction (real-time PCR), enzyme-linked immunosorbent assay (ELISA), and immunofluorescence were used to detect the differentiation of the RG-like cells into.. Read More»
  DOI: 10.4172/2157-7099.1000312

  Abstract PDF