Molecular Biology: Open Access

Hamuryudan V

Publications

• Research Article
  hsa-miR-149-5p Diminish MAPK and PI3K/Akt Signalling Pathways through Down-regulation of ERbB3 in SLE Patients
  Author(s): Ghods FJ, Sarikaya AT, Arda N and Hamuryudan VGhods FJ, Sarikaya AT, Arda N and Hamuryudan V
  
  Introduction: In Systemic Lupus, miRNAs construct a substantial layer of post-transcriptional gene expression regulation. Availability of sensitive and specific methods for their detection makes them candidates for potential biomarker discovery. Here we compared miRNAs particular to SLE patients with healthy controls.Methods: Total RNA and miRNAs were isolated from blood and serum of 16 SLE patients and 8 healthy controls for microarray assays. Potential target genes were predicted and interrogated with mRNA profiling data. BLAST alignment analysis was done between differentially expressed miRNAs and predicted target genes. Microarray results were confirmed by QRT-PCR.Results: 10 miRNAs were differently expressed in SLE patients of which, has-miR-149-5p was up-regulated about 8.5 fold. Among predicted targets only ERbB3 ap.. Read More»
  DOI: 10.4172/2168-9547.1000224

  Abstract PDF