Biosensors & Bioelectronics

Hani A Alhadrami

Publications

• Research Article
  Validation of SOS-lux Microbial Biosensors for Mutagenicity Assessment: Mitomycin-C as a Model Compound
  Author(s): Hani A Alhadrami and Graeme I PatonHani A Alhadrami and Graeme I Paton
  
  Human health protection requires relating the bioaccessible concentration of a mutagen with the corresponding likely harm that could be caused through exposure. This requires designating the target receptor in need of protection, and a quantitative understanding of the likely pathways for mutagen availability. In this study, young children were selected as target receptors because of their tendency to directly ingest soils. Most data used to characterise a chemical mutagenicity has been extrapolated from rat-based assays using chemical ingestion or direct injection procedures. Mitomycin C was selected as a relevant model compound and extracted using an established in vitro digestion technique. A range of mutagenic bioassays (i.e. SOS-lux based microbial biosensors and Salmonella mutagenicity assay) were calibrated and optimised in aqueous samples, before being applied to soil extracts.. Read More»
  DOI: 10.4172/2155-6210.1000142

  Abstract PDF