Journal of Material Sciences & Engineering

Harou Sugi

Publications

• Research Article
  Effect of Antibodies to Myosin Head on the Development of Rigor Tension and Stiffness in Skinned Muscle Fibers
  Author(s): Ohno T, Abe T and Harou SugiOhno T, Abe T and Harou Sugi
  
  Using three antibodies to myosin head, attaching to (1) distal region and (2) proximal region of myosin head catalytic domain, and (3) to myosin head lever arm domain, respectively, we have shown definite differences between in vitro actin-myosin sliding and muscle contraction. In the present study, we studied the effect of these antibodies on the development of rigor tension and stiffness in single skinned muscle fibers at pCa>9. To form rigor actin-myosin linkages, myosin heads should override tropomyosin, covering myosin-binding sites on actin, and to detach antibodies from them. Despite their different attachment sites in myosin head, all these antibodies slowed down development of rigor tension and stiffness with or without changing their peak values. The rigor tension versus stiffness relation was highly variable, suggesting that the rigor tension reflects the sum of tension .. Read More»
  DOI: 10.4172/2169-0022.1000435

  Abstract PDF