Journal of Cytology & Histology

Haruhiko Yoshioka

Publications

• Expert Review
  New Method to Restore Fading Papanicolaou Specimen Using an Optical Clearing Agentx
  Author(s): Kiyotada Washiya, Makoto Sakurai, Saki Furutate, Estuko Okusawa, Shigeharu Hatakeyama, Haruhiko Yoshioka and Jun WatanabeKiyotada Washiya, Makoto Sakurai, Saki Furutate, Estuko Okusawa, Shigeharu Hatakeyama, Haruhiko Yoshioka and Jun Watanabe
  
  Cytological specimens are used only once for staining, and cannot be reused after preservation in paraffin blocks. If a technique is developed allowing restaining of precious/rare specimens or those for education, which have been discolored due to long-term preservation, to restore their former states, it will be extremely useful for the reuse of specimens. We performed this study to determine whether discolored specimens can be restored to their former condition by applying an optical clearing agent. For restaining, 131 Papanicolaou (Pap) specimens prepared from 13 to more than 30 years ago were used. Using an optical clearing agent (SCALEVIEW-A2), Pap restaining, endurance tests of the optical clearing agent by repeated use, and immunostaining were performed. After treatment with the optical clearing agent for5-10 minutes, discolored cytological specimens could be stably restained j.. Read More»
  DOI: 10.4172/2157-7099.1000219

  Abstract PDF