Journal of Cytology & Histology

Haruhiko Yoshioka

Publications

• Research Article
  Usefulness of Evaluation of Nuclear Color by Visible-Microscopic Spectroscopy for Objective Differentiation between Non-Cancer and Cancer Cells Prepared Using Liquid-Based Cytology
  Author(s): Haruhiko Yoshioka, Keita Hoshiai, Toshiya Nakamura, Kayo Horie, Kiyotada Washiya and Jun WatanabeHaruhiko Yoshioka, Keita Hoshiai, Toshiya Nakamura, Kayo Horie, Kiyotada Washiya and Jun Watanabe
  
  Objective: The aims of this study were to investigate the usefulness of color evaluation of the nuclear region using visible-microscopic spectroscopy (Vis-MS) and to clarify whether it can serve as an index to distinguish cancer cells in liquid-based cytology (LBC). Vis-MS is a spectral analysis technique widely used for absorption and fluorometric analyses in the analytical chemistry field. Vis-MS has been applied to histological diagnosis, but only a few studies on its application to cytology have been performed, and no investigations have been performed for LBC, which is expected to become widely used in Japan. Study design: Using culture cell lines of non-cancer cells and cancer cells, transmittance at 530 nm (maximum absorption wavelength of eosin), 580 nm (hematoxylin), and 630 nm (light green), and 530 nm/580 nm and 630 nm/580 nm transm.. Read More»
  DOI: 10.4172/2157-7099.1000308

  Abstract PDF