Journal of AIDS & Clinical Research

Hayes KC

Publications

• Research Article
  Oxidative Damage is not a Major Contributor to AZT-Induced Mitochondrial Mutations
  Author(s): Adam E Osborne, J Aquiles Sanchez, Lawrence J Wangh, Ravigadevi Sambanthamurthi and Hayes KCAdam E Osborne, J Aquiles Sanchez, Lawrence J Wangh, Ravigadevi Sambanthamurthi and Hayes KC
  
  Addition of clinically-relevant levels of 3′-Azido-3′-deoxythymidine (AZT) to cultured HepG2 cells increases the number of reactive radical species (reactive oxygen and nitrogen species [ROS and RNS]) as well as random mutations in mitochondrial DNA (mtDNA). Co-treatment of AZT-exposed cells with palm fruit juice (PFJ) mitigates AZT mutagenesis. These findings suggest that AZT-dependent mtDNA damage resulted from increased reactive species and that PFJ, a known anti-oxidant, mitigated such damage by decreasing the levels of these species. The present report tests the predictions that (1) PFJ mitigates AZT mutagenesis by reducing the burden of AZT-induced reactive species, and (2) AZT-induced mutations in mtDNA should predominantly consist of G → T and C → A substitutions characteristic of DNA oxidative damage. Levels of reactive species and mitoc.. Read More»
  DOI: 10.4172/2155-6113.1000441

  Abstract PDF