Biosensors & Bioelectronics

Hilber W

Publications

• Research Article
  Reflection Coefficient S11 Related Measurement System for Label-Free Cell Seeding Analysis and Drug Testing in a Three-Dimensional (3D) Cell Culture Model
  Author(s): Lornejad-Schäfer MR, Hilber W and Christine SchäferLornejad-Schäfer MR, Hilber W and Christine Schäfer
  
  Context: With regard to two-dimensional (2D) cell cultures in flat dishes, most of the three-dimensional (3D) cell cultures have advantages and gain importance in life sciences. But, their higher complexity requires adaptation of existing methods or different measurement systems in order to achieve accurate results. Bio-Impedance spectroscopy (Bio-IS) is a label- free and non-destructive method that is considered for analysis of 3D cell models. Purpose: We design a new Bio-IS measurement system for a 3D liver cell model to assess cell seeding and drug effects. Experiment Methods: We measured reflection coefficient S11 with differently (disc- and needle-like) shaped electrodes, which are in defined contact with the 3D liver cell model, metabolic activity (MTT test), and cytotoxicity (LDH assay). Results: We demonstrate .. Read More»
  DOI: 10.4172/2155-6210.1000151

  Abstract PDF