Molecular Biomarkers & Diagnosis

Hisashi Narimatsu

Publications

• Research Article
  Identification of Core Proteins Carrying the Sialyl Lewis a Epitope in Pancreatic Cancers
  Author(s): Yoshitoshi Hirao, Satoshi Ogasawara, Akira Togayachi, Yu-ki Matsuno, Makoto Ocho, Keishi Yamashita, Masahiko Watanabe, Shoji Nakamori, Yuzuru Ikehara and Hisashi NarimatsuYoshitoshi Hirao, Satoshi Ogasawara, Akira Togayachi, Yu-ki Matsuno, Makoto Ocho, Keishi Yamashita, Masahiko Watanabe, Shoji Nakamori, Yuzuru Ikehara and Hisashi Narimatsu
  
  Identification of core proteins carrying the CA19-9 (carbohydrate antigen, sialyl Lewis a) epitope from various tissues will improve the diagnosis of pancreatic cancer in terms of specificity and sensitivity. In this study, we attempted to identify sialyl Lewis a-carrier proteins specifically expressed in pancreatic cancer. Pancreatic cancer is difficult to detect in the early stages of the disease, resulting in a high level of mortality. Therefore, in order to determine the correct course of treatment, it is vital to distinguish cancer from obstruction of the bile duct or other diseases. Our strategy to identify the carrier proteins was as follows: glycoproteins carrying sialyl Lewis a antigen were enriched from pancreatic cancer cell lines using anti-sialyl Lewis a antibody and then subjected to Peptide Mass Fingerprinting analysis. Based on these studies we identified nine glycopro.. Read More»
  DOI: 10.4172/2155-9929.1000124

  Abstract PDF