Journal of Computer Science & Systems Biology

Holger M. Becker

Publications

• Research Article
  A Kinetic Model of the Monocarboxylate Transporter MCT1 and its Interaction with Carbonic Anhydrase II
  Author(s): Joachim Almquist, Patrick Lang, Dieter Prätzel- Wolters, Joachim W. Deitmer, Mats Jirstrand and Holger M. BeckerJoachim Almquist, Patrick Lang, Dieter Prätzel- Wolters, Joachim W. Deitmer, Mats Jirstrand and Holger M. Becker
  
  The enzyme carbonic anhydrase isoform II (CAII), catalyzing the hydration and dehydration of CO2, enhances transport activity of the monocarboxylate transporter isoform I (MCT1, SLC16A1) expressed in Xenopus oocytes by a mechanism that does not require CAII catalytic activity. In the present study, we have investigated the mechanism of the CAII induced increase in transport activity by using electrophysiological techniques and mathematical modeling of the MCT1 transport cycle. The model consists of six states arranged in cyclic fashion and features an ordered, mirrorsymmetric, binding mechanism, where binding and unbinding of the proton to the transport protein is considered to be the rate limiting step under physiological conditions. An explicit rate expression for the substrate flux is derived using model reduction techniques. By treating the pools of in.. Read More»
  DOI: 10.4172/jcsb.1000066

  Abstract PDF