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Malaysia
Research Article
Bioprocessing of Agro-Residual Wastes for Optimisation of Xylanase Production by Aspergillus brasiliensis in Shake Flask Culture and Its Scaling up Elucidation in Stirred Tank Bioreactor
Author(s): Hooi Ling Ho and Jee Hsiung PhangHooi Ling Ho and Jee Hsiung Phang
Objective:Xylanase is a hydrolytic enzyme that breaks down backbone of xylan into xylose sugars. This extracellular enzyme possesses many applications in pulp and paper, food and beverage industries. Malaysia as an agricultural country contributes more than 5 million tons of agricultural wastes per year, thus, it is feasible to utilize agro-residual wastes to produce xylanase.
Methods:In the present study, Aspergillus brasiliensis ATCC 16404 was investigated for the production of xylanase in shake flask culture using different agro-residual wastes including wheat bran, sawdust, rice bran, sugarcane bagasse, barley husks, soybean hulls and palm kernel cake. A. brasiliensis was cultured at initial pH 6.5, agitation speed of 150 rpm at 30°C for 144 h. Thereafter, the optimised carbon sources obtained from earlier experiments were further elucidated in stirred tank bioreacto.. Read More»
DOI:
10.4172/2376-0214.1000148