Molecular Biomarkers & Diagnosis

J Aquiles Sanchez

Publications

• Research Article
  Single-Tube Mutation Scanning of the Epidermal Growth Factor Receptor Gene using Multiplex LATE-PCR and Lights-On/Lights-Off Probes
  Author(s): Shana M Tetrault, John E Rice, Lawrence J Wangh and J Aquiles SanchezShana M Tetrault, John E Rice, Lawrence J Wangh and J Aquiles Sanchez
  
  Background: Numerous mutations in exons 18-21 of the epidermal growth factor receptor (EGFR) gene determine the response of many patients with Non-Small Cell Lung Carcinoma (NSCLC) to anti-EGFR Tyrosine Kinase Inhibitors (TKIs). This paper describes a single closed-tube assay for simultaneous mutational scanning of EGFR exons 18-21 using Linear-After-The-Exponential (LATE)-PCR and Lights-On/Lights-Off probes. Methods: The assay first co-amplifies all four exons as separate single-stranded DNA products using LATEPCR. The amplicons are then interrogated at endpoint along their length using sets of Lights-On/Lights-Off probes of a different color for each exon. The four resulting fluorescent signatures are unique for each underlying DNA sequence. Every mutation in a target potentially alters its unique fluorescent signature thereby revealing th.. Read More»
  DOI: 10.4172/2155-9929.1000175

  Abstract PDF