Journal of Cytology & Histology

Jacek Baszak

Publications

• Research Article
  Distinct IRAK4 Expression in Normal and B-CLL Lymphocytes
  Author(s): Halina Antosz, Joanna Sajewicz, Barbara Marzec-Kotarska, Dorota Choroszynska, Iwona Hus, Malgorzata Jargiello-Baszak and Jacek BaszakHalina Antosz, Joanna Sajewicz, Barbara Marzec-Kotarska, Dorota Choroszynska, Iwona Hus, Malgorzata Jargiello-Baszak and Jacek Baszak
  
  Toll-like receptors (TLR) signaling plays an important role in the B cell biology. It induces the maturation, proliferation and antibody production in response to pathogen recognition. Interleukin-1 Receptor-associated Kinase-4 (IRAK-4) is TLR downstream molecule which is of strategic importance in starting a cascade of NF-kappaB (Nuclear factor-kappaB) and MAPK (Mitogen-activated protein kinase) intracellular signaling pathways. The aim of the study was to identify the differences in IRAK-4 expression in normal and chronic lymphocytic leukemia (CLL) lymphocytes. We have also studied the IRAK-4 expression alteration upon TLR2/4 ligand stimulation in leukemic and normal B cells. We carried out Real-Time PCR and Western blot analysis of IRAK4 mRNA (RQ) and protein expression. The study were performed on the isolated CD19+ normal B lymphocytes and CLL cells (CD5+CD19+) before and 30 min .. Read More»
  DOI: 10.4172/2157-7099.1000228

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