Journal of Cytology & Histology

Jesús Martínez-Hernández

Publications

• Mini Review
  Identification of Apoptotic Cells by Means of Lectin Histochemistry: State of the Art Review
  Author(s): Vicente Seco-Rovira, Esther Beltrán-Frutos, Concepción Ferrer, Jesús Martínez-Hernández and Luis M. PastorVicente Seco-Rovira, Esther Beltrán-Frutos, Concepción Ferrer, Jesús Martínez-Hernández and Luis M. Pastor
  
  Apoptosis is a cellular phenomenon of great importance in the cellular homeostasis of tissues and organs. For many years diverse techniques have been developed for the identification and differentiation of normal and apoptotic cells, but only in cells isolated in cultures as in histology sections. These techniques are based on the modifications that cells suffer during the process of apoptosis. One of these changes is linked with modifications of glycoconjugates in the glycocalix of cells, which allows the use of lectins for identification of these apoptotic cells. In this review, we first present the data obtained to date with this approach for the detection of apoptotic cells with lectins, both using flow cytometry and, especially, cytochemical methods. Secondly we comment on the results obtained in the detection of apoptotic cells with classical lectin histochemistry using histolog.. Read More»
  DOI: 10.4172/2157-7099.1000309

  Abstract PDF