Vitamins & Minerals

John B. Porter

Publications

• Research Article
  Cytotoxicity and Apoptogenic Activity of A Novel Synthetic Iron Chelator 1-(N-Acetyl-6-Aminohexyl)-3-Hydroxy-2-Methylpyridin-4-One (CM1) In Human Leukemic Cells
  Author(s): Nittaya Chansiw, Kanjana Pangjit, Wirote Tuntiwechapikul, Chada Phisalaphong, Suthat Fucharoen, John B. Porter and Somdet SrichairatanakoolNittaya Chansiw, Kanjana Pangjit, Wirote Tuntiwechapikul, Chada Phisalaphong, Suthat Fucharoen, John B. Porter and Somdet Srichairatanakool
  
  An interruption of the iron metabolism with chelators can lead to a significant inhibition of cancer cell growth. 1-(N-acetyl-6-aminohexyl)-3-hydroxy-2-methylpyridin-4-one or CM1, is a novel synthetic bidentate iron chelator which was successfully synthesized by our group. We have studied the characteristics and iron-chelating activity of this compound. Nevertheless, the anti-cancer activity of the chelator is largely unknown. In this study, we demonstrated the cytotoxicity and apoptogenic activity of CM1 against human leukemic cell lines-HL-60 and U937. 3-(4,5-Dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed for the cytotoxicity study. The results showed that CM1 inhibited the cell growth and metabolic activity of the leukemic cells. Flow cytometric analysis clearly demonstrated the dose and time-response of CM1-induced apoptosis in these two cells. CM.. Read More»
  DOI: 10.4172/vms.1000114

  Abstract PDF