Molecular Biomarkers & Diagnosis

John F. Palma

Publications

• Research Article
  Performance Characteristics of a PCR Assay for the Detection of KRAS Mutations in Formalin-Fixed Paraffin-Embedded Tissue Samples of Non-Small Cell Lung Cancer
  Author(s): Sung Lee, Jianli Cao, Theresa May, Jingchuan Li, Lilia Corona, Nitta Lee, Yiqiao Wu, Carrie Wong, Kelli DeMartin, Victoria H. Brophy, Stephen Soviero and John F. PalmaSung Lee, Jianli Cao, Theresa May, Jingchuan Li, Lilia Corona, Nitta Lee, Yiqiao Wu, Carrie Wong, Kelli DeMartin, Victoria H. Brophy, Stephen Soviero and John F. Palma
  
  Introduction: Approximately 25% of non-small cell lung cancer (NSCLC) tumors contain mutations in KRAS. These tumors are insensitive to therapy directed against the epidermal growth factor receptor and appear to be resistant to adjuvant chemotherapy. The current study demonstrates the performance of the cobas® KRAS Mutation Test (cobas test), a TaqMelt polymerase chain reaction (PCR) assay designed to detect 19 mutations in codons 12, 13, and 61. Methods: To reflect real-world testing conditions, the study used formalin-fixed, paraffin-embedded tissue (FFPET) NSCLC samples for the predominant mutations. DNA blends of cell lines and plasmids were used where FFPET samples were not available. Results: In the limit of detection study, a correct mutation call rate of ≥95% was obtained with approximately 5% mutant.. Read More»
  DOI: 10.4172/2155-9929.1000246

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