Molecular Biology: Open Access

Jonathan D. Chesnut

Publications

• Research Article
  An Alternate Method for Efficient Delivery of Catalyzing Enzymes
  Author(s): Uma Lakshmipathy, Andrew Fontes, Michael Poderycki and Jonathan D. ChesnutUma Lakshmipathy, Andrew Fontes, Michael Poderycki and Jonathan D. Chesnut
  
  Improved cellular engineering tools have enabled scientists to modify genomic sequences at a new pace for a variety of applications. However, in order to create more accurate tools for site specific targeting and editing, improved delivery systems for modifying enzymes are required. These enzymes allow end users to disrupt, insert or edit genes utilizing sequence specific homology. Current applications require co-transfection of an engineering plasmid in tandem with the vector carrying the modifying enzyme. However, co-transfection can negatively affect transfection efficiencies as well as increase probability of randomly integrating DNA. Here we report the transient delivery of catalyzing enzymes via BacMam non-integrating virus at high efficiency. Baculovirus is a non-infectious delivery system that can transduce a variety of mammalian cells. We have shown improved delivery of NLS-C.. Read More»
  DOI: 10.4172/2168-9547.1000112

  Abstract PDF