Colombia
Research Article
Isolation of Catechin and Gallic Acid from Colombian Bark of Pinus patula
Author(s): Rodrigo A Sarria-Villa, José A Gallo-Corredor and Martha Isabel PáezRodrigo A Sarria-Villa, José A Gallo-Corredor and Martha Isabel Páez
Pine bark is a rich source of natural polyphenols, compounds which have attracted increasing attention in the fields of nutrition, health and medicine. Extractive components include large amounts of phenolic compounds. The ethanolic extract was obtained from Pinus patula bark grown in the forests of the Cauca Department in Colombia South America. The Gallic acid, catechin, epicatechin and procyanidin B2 contents in ethanolic extract were 3.12, 1.99, 0.80, and 0.71%, respectively, these compounds were determined using HPLC with UV-Vis detection. Ethanolic extract was divided using column chromatography to obtain an ethanol-soluble fraction with tannins and phlobaphenes as well as an ethanol-insoluble fraction mainly composed of Phenolic acids. Catechin and Gallic acid finally isolated were characterized using UV-Vis, IR, NMR, 13C, 1 H, HSQC, HMBC and GC-MS spectrometry... Read More»
DOI:
10.4172/2150-3494.1000174
Chemical Sciences Journal received 912 citations as per Google Scholar report