University of Alcalá de Henares,
Madrid-28871
Spain
Research Article
SQPrimer: The Utility of Designing Homologous Primers for the Genetic Analysis Based on the PCR
Author(s): Julio Perez-MarquezJulio Perez-Marquez
Objective: Most bioinformatics applications that design primers for the technique of the PCR analyze one single sequence of DNA as template; only a few applications process various nucleotide sequences. A common feature of existing software is that produces primers that are unique for each template, which is partially useful in the genomic analysis. The objective was to create an application able to find the primers that are identical in multiple nucleotide sequences and the primers that are unique to each sequence.
Methods: I applied object-oriented programming using the C++ Builder 2009 to implement algorithms that find particular short strings (primers) in nucleotide sequences. The software is a set of applications with a simple design that serves as a didactic tool to find and analyze primers. To test the application, I .. Read More»
DOI:
10.4172/jcsb.1000162
Journal of Computer Science & Systems Biology received 2279 citations as per Google Scholar report