Biosensors & Bioelectronics

Jun Kobayashi

Publications

• Research Article
  Effect of Cell Density on Reproducibility in a Cell-Based Biosensor Using a Microwell Array
  Author(s): Satoshi Migita, Kazuyoshi Itoga, Jun Kobayashi, Teruo Okano and Akiyoshi TaniguchiSatoshi Migita, Kazuyoshi Itoga, Jun Kobayashi, Teruo Okano and Akiyoshi Taniguchi
  
  Combinations of living cell-based biosensors and microdevices are attractive tools for real-time monitoring of gene expression profiling in a small population of cells involving small amount of analytes. However, due to the heterogeneous responsiveness of cells, cell-based biosensors have poor reproducibility and a low signal-to-noise (S/N) ratio. Previously, we constructed a “sensor cell”, a GFP reporter cell line containing an engineered Heart Shock Protein 70B’ promoter generated by stably transfecting mouse NIH/3T3 cells. In this study, we manipulated the cell density to overcome the lower signal and poor reproducibility using the sensor cells. We found that a cell density of 2 x 105 cells/cm2 provides good responsiveness of sensor cells that appears to be related to the G0/G1 phase of cell cycle. However, higher cell densities had a negative effect for on sensor.. Read More»
  DOI: 10.4172/2155-6210.1000118

  Abstract PDF