Journal of Bioprocessing & Biotechniques

Junaid Muneer Raja

Publications

• Research Article
  Study on Interactions between Fed-Batch and Batch Operating Parameters for the Development of Monoclonal Antibody Fed-Batch using Design of Experiments
  Author(s): Junaid Muneer Raja, Nurina Anuar, Badarulhisam Abdul Rahman and Jamaliah Md JahimJunaid Muneer Raja, Nurina Anuar, Badarulhisam Abdul Rahman and Jamaliah Md Jahim
  
  In conventional fed batch process development approaches, batch operating parameters (such as pH, temperature, seeding density, dissolved oxygen concentration) are kept constant and only feeding parameters such as feeding time, post-feed concentration are manipulated. The batch and fed batch operating parameters are assumed to be independent of each other. This approach to process development ignores any interactions that might exist between the batch and fed-batch operating parameters are therefore not evaluated. However in a complex bioprocess, none of the factors affecting the process can be assumed to be independent of each other and mutually exclusive. Therefore in this study an attempt was made to study the interaction between fed-batch operating parameter-post feed glucose concentration (A) and batch operating parameters- seeding density (B), temperature (C), and dissolved oxyg.. Read More»
  DOI: 10.4172/2155-9821.1000165

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